HPLC chromatography column Aluspher® 100 RP

Merck’s Aluspher® RP-select B HPLC column is an alkali-stable reversed-phase stationary phase designed for the chromatographic separation of neutral, basic and acidic compounds under neutral conditions. Due to its stability in the pH range of 2-12, Aluspher® RP-select B allows the use of basic eluents, such as NaOH. This suppresses the ionization of basic compounds, and thus avoids peak tailing. Furthermore, advanced formulation technology has enabled the production of spherical alumina particles as a base for Aluspher® 100 RP-select B. The combination of alumina with alkaline eluents has led to new applications in HPLC. Mobile phase Acetonitrile/Water 35/65 (v/v) Flow rate 1.0 ml/min Detection UV 220 nm Temperature - Room temperature Sample - 1. Levamisole 2. 5-Methyl-5-phenyldantoin (MPH) 3. 5-Phenyl-5-(2-pyridyl)-hydantoin (PPH) 4. 5-(p-Methylphenyl)-5-phenylhydantoin (MPH) 5. Diazepam