Solution reagent 091689649

enzymetrypsin-EDTAHBSS

Alternate Names - Trypsin (1X), Trypsin-EDTA solution Application Notes - Trypsin-EDTA solutions are used to release adherent cells from culture surfaces. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Ethylenediaminetetracetic acid (EDTA), a chelating agent, is added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cellto-cell adhesion and obscure the peptide bonds on which trypsin acts. Base Catalog Number - 1689649 CAS # - 9002-07-7 (trypsin) Concentration - 0.25% (w/v) solution | 0.25% Trypsin (1:250) and 1mM EDTA In Modified HBSS Without Ca++ and Mg++ Format - Solution Formulation Details - Liquid | 0.25% Trypsin (1:250) and 1mM EDTA In Modified Hbss Without Ca++ and Mg++ Media Type - Mammalian Media Molecular Weight - Approximately 23;000 (trypsin) Pack Size - 100 mL pH - 7.0 - 7.3 Source - Porcine (for trypsin) Specific Activity - 1 TAME unit = 19.2 USP or NF units = 57.5 BAEE Units Specificity - Specificity: The protease activity of trypsin is highly specific toward positively charged side chains with lysine and arginine. Forms complexes with α2-macroglobulin. Can be used in the isolation of intact; detergent-free phycobilisomes and in the hydrolysis/condensation of carboxylic ester bonds.