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Describtion -
This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human β-NGF. The Human β-NGF ELISA is for research use only. Not for diagnostic or therapeutic procedures.Programmed cell death protein 1, also known as PD-1 and CD279, is a protein that in humans is encoded by the PDCD1 gene. PD-1 is a cell surface receptor that belongs to the immunoglobulin superfamily and is expressed on T cells and pro-B cells. In vitro, ligation of PD-1 inhibits TCR-mediated T cell proliferation and production of IL-1, IL-4, IL-10, and IFN-γ. In addition, PD-1 ligation also inhibits BCR mediated signaling. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases. The inhibitory effect of PD-1 is accomplished through a dual mechanism of promoting apoptosis (programmed cell death) in antigen specific T-cells in lymph nodes while simultaneously reducing apoptosis in regulatory T cells.
Components -
96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody against human PD-1
Human PD-1 Standard, lyophilized
PD-1 Detect Antibody
Standard Diluent
Assay Buffer (10×)
Substrate (TMB)
Washing Buffer (20×)
Adhesive Films
Sensitivity - 8.62 pg/ml
Standard Curve Range - 93.75-6000 pg/ml
Spike Recovery Range - 95%-118%
Mean Spike Recovery - 107%
CV of Intra plate - 4.8 % - 4.9 %