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Components -
96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody against human Betacellulin
Human Betacellulin Standard, lyophilized
Betacellulin Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Adhesive Films
Describtion -
This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human β-NGF. The Human β-NGF ELISA is for research use only. Not for diagnostic or therapeutic procedures.Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
Storage - 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition - 4℃
Sensitivity - 0.45 pg/ml
Standard Curve Range - 3.91-250 pg/ml
Spike Recovery Range - 89%-115%
Mean Spike Recovery - 103%
CV of Intra plate - 4.1 % - 4.9 %
CV of Inter plate - 0.6% - 3.4%