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Components -
96-well polystyrene microplate coated with a monoclonal antibody against C-peptide
Human C-peptide Standard, lyophilized
C-peptide Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
Describtion -
C-Peptide, or connective peptide, is secreted by Pancreatic beta cell. It shares a common proinsulin with the mature Insulin. It is a short 31-amino-acid polypeptide that connects insulin's A-chain to its B-chain in the proinsulin molecule. C-peptide has been shown to bind to the surface of a number of cell types such as neuronal, endothelial, fibroblast and renal tubular, at nanomolar concentrations to a receptor that is likely G-protein-coupled.
C-peptide exerts vascular anti-inflammatory effects via the downregulation of endothelial cell surface adhesion molecules, the reduction of inflammatory cytokine production, and the protection of vascular smooth muscle cells from detrimental Insulin effects. C-peptide administration in animals that had C-peptide deficiency (type 1 model) with nephropathy improves renal function and structure; it decreases urinary albumin excretion and prevents or decreases diabetes-induced glomerular changes secondary to mesangial matrix expansion.
Shipping Condition - 4℃
Sensitivity - 2.86 pg/ml
Standard Curve Range - 39.06-2500 pg/ml
Spike Recovery Range - 75%-123%
Mean Spike Recovery - 95%
CV of Intra plate - 3.8 % - 7.4 %