Microbiology test kit 70-EK1351-96

for genesserumplasma

Welcome to buy the high quality and sanitary 96 wells human il-6 elisa kit with our professional manufacturers and suppliers. We can assure of you of the high quality and competitive price of our ELISA kit for procedure. PRINCIPLE OF THE ASSAY - Human GDNF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human GDNF in the samples. An antibody specific for human GDNF has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for GDNF, and GDNF present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of GDNF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader. Describtion - Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central and peripheral nervous systems at different stages of their development. The functional human GDNF ligand is a disulfide-linked homodimer, of two 15 kDa polypeptide chains called monomers. GDNF is produced by Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells.