Microbiology test kit 70-EK1345-96

serumplasmacell

Welcome to buy the high quality and sanitary 96 wells human il-6 elisa kit with our professional manufacturers and suppliers. We can assure of you of the high quality and competitive price of our ELISA kit for procedure. PRINCIPLE OF THE ASSAY - Human Endostatin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Endostatin in the samples. An antibody specific for human Endostatin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Endostatin, and Endostatin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Endostatin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader. Describtion - Endostatin is a 20 kDa proteolytic fragment of the C-terminal, non-collagenous (NC1) domain of type XVIII Collagen, which is an endogenous angiogenesis inhibitor. It was originally identified as a factor produced by murine hemangioendothelioma cells that could specifically inhibit endothelial cell proliferation and angiogenesis. It inhibits tumor growth and impairs blood vessel maturation in wound healing. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization.