Microbiology test kit 70-EK1323-96

serumplasmacell

Welcome to buy the high quality and sanitary 96 wells human il-6 elisa kit with our professional manufacturers and suppliers. We can assure of you of the high quality and competitive price of our ELISA kit for procedure. PRINCIPLE OF THE ASSAY - Human BMP-4 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human BMP-4 in the samples. An antibody specific for human BMP-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and BMP-4 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for BMP-4 is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of BMP-4 bound in the initial step. The color development is stopped and the intensity of the color is measured. Describtion - BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. In general, BMPs are implicated in embryogenesis and morphogenesis of various tissues and organs. BMP-4, also known as Bone morphogenetic protein 4, forms disulfide-linked homodimer. It plays an important role in the onset of endochondral bone formation, dorsal/ventral patterning and has also been implicated in the commitment of embryonic mesodermal cells to a hematopoietic fate in a number of systems.