Microbiology test kit 70-EK1317-96

serumplasmacell

Welcome to buy the high quality and sanitary 96 wells human il-6 elisa kit with our professional manufacturers and suppliers. We can assure of you of the high quality and competitive price of our ELISA kit for procedure. PRINCIPLE OF THE ASSAY - Human ACE ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human ACE in the samples. An antibody specific for human ACE has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for ACE, and ACE present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of ACE bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader. Describtion - Angiotensin I Converting Enzyme (ACE), also known as peptidyl-dipetidase A, is a zinc metallopeptidase important for blood pressure control and water and salt metabolism. ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu dipeptide, this results in an increase of the vasoconstrictor activity of angiotensin. It is also able to inactivate bradykinin, a potent vasodilator. ACE and ACE-2, two cell surface proteases, are important regulators of the renin-angiotensin system (RAS), which plays a key role in maintaining blood pressure homeostasis and fluid salt balance in mammals.