Microbiology test kit 70-EK1364-96

serumplasmacell

Welcome to buy the high quality and sanitary 96 wells human il-6 elisa kit with our professional manufacturers and suppliers. We can assure of you of the high quality and competitive price of our ELISA kit for procedure. PRINCIPLE OF THE ASSAY - Human Notch-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Notch-1 in the samples. An antibody specific for human Notch-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Notch-1, and Notch-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Notch-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader. Describtion - The Notch signaling pathway is a ubiquitous pathway that controls a variely of processes during development. The Notch family comprises a group of highly conserved proteins that function both as cell surface receptors and direct regulators of gene transcription. It plays a significant role in the regulation of cell proliferation,apoptosis,differentiation,as well as a series of physiological and pathological processes.In addition,it has a vital effect in cancer occurrence and development.