Microbiology test kit 70-EK1342-96

serumplasmacell

Welcome to buy the high quality and sanitary 96 wells human il-6 elisa kit with our professional manufacturers and suppliers. We can assure of you of the high quality and competitive price of our ELISA kit for procedure. PRINCIPLE OF THE ASSAY - Human E-Cadherin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human E-Cadherin in the samples. An antibody specific for human E-Cadherin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for E-Cadherin, and E-Cadherin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of E-Cadherin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader. Describtion - E-Cadherin, also known as Cadherin-1, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration. E-Cadherin is a single-pass transmembrane protein on the plasma membrane.