Microbiology test kit 70-EK1291-96

for antibodiesserumplasma

Welcome to buy the high quality and sanitary 96 wells human il-6 elisa kit with our professional manufacturers and suppliers. We can assure of you of the high quality and competitive price of our ELISA kit for procedure. PRINCIPLE OF THE ASSAY - Human CCL25/TECK ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human CCL25 in the samples. An antibody specific for human CCL25 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and CCL25 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for CCL25 is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of CCL25 bound in the initial step. The color development is stopped and the intensity of the color is measured. Describtion - Chemokine C‐C motif ligand 25 (CCL25), also known as thymus‐expressed chemokine (TECK), is a chemoattractant that is produced by stromal cells in the thymus and epithelial cells of the small intestine. CCL25 induces the homing of several lymphocyte populations to the small intestine, including Integrin a4b7+ gamma/delta T cells, Integrin aEb7+ CD8+ T cells, and IgA-producing plasma cells. CCL25 operates following binding to the chemokine receptor CCR9.CCL25 attracts CCR9+ monocytes and macrophages in rheumatoid arthritis and CCR9+ macrophages in atherosclerosis.