This highly sensitive, ready-to-use PCR kit is available in lyophilized format (freeze-drying process) for long-term storage. The kit can be transported and stored at room temperature and is stable for one year. Each tube of premix contains all of the reagents needed for the PCR amplification, including Reverse-transcriptase, Taq polymerase, primers, probes, and dNTPs substrates. It only need add 13ul distilled water and 5ul extracted RNA template , then it can be run and amplified on the PCR instruments.
The qPCR machine should meet the following requirements:
1. Fit 8 strip PCR tube volume 0.2 ml
PCR-platforms:
7500Real-Time PCR System, Biorad CF96, iCycler iQ™ Real-Time PCR Detection System, Stratagene Mx3000P, Mx3005P
The difficulty of cold chain transportation of Novel Coronavirus nucleic acid detection reagent
When conventional nucleic acid detection reagents are transported in long distance, the (-20±5) ℃ cold chain storage and transportation is required to ensure the bioactive of enzyme in the reagents remain active. To make sure that the temperature reaches the standard, several kilograms of dry ice are needed for each box of nucleic acid testing reagent even less than 50g , but it can only last for two or three days. On the perspective of industry practice, the actual weight of reagents issued by manufacturers is less than 10% (or far less than this value) of the container. Most of the weight comes from dry ice, ice packs and foam boxes, so the transportation cost is extremely high.
Extremely strong heat stability: it can with stand treatment at 56℃ for 60 days, and the morphology and performance of the reagent remain unchanged.