NadPrep DNA Library Preparation Module v2 is designed for the preparation of high-quality libraries from double-stranded DNA (dsDNA) on Illumina® & MGI platforms. This A-T ligation-based kit offers a stable and efficient library preparation solution for ap plications including whole genome sequencing of 1‒500 ng DNA and hybridization capture based targeted sequencing. Comp ared to the v1 version, the upgraded kit has improved the library conversion efficiency and better tolerance for low-quality sam ples. Consequently, it can enhance library complexity while maintaining high fidelity.
Product details
Product Feature
● Fast and Convenient
Complete the entire process of whole-genome library preparation within 3 hr
● Stable and Efficient
Higher library yield
Higher library conversion rate
Low GC bias
Higher Library Complexity
● Flexible Compatibility
Flexible selection of multiple types of adapters to accommodate both MGI and Illumina platforms
Compatible with input amounts ranging from 1 ng to 500 ng
Compatible with various sample types such as gDNA, FFPE DNA, cfDNA, etc
Compatible with liquid-phase hybridization capture
Can RNA samples be directly used for library preparation with this kit?
No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation.
What is the recommended input range? How to handle inputs exceeding this range?
This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency.