DNA library preparation reagent kit NadPrep EZ

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NadPrep EZ DNA Library Preparation Kit v2 is designed for preparation of high-quality sequencing libraries from double-stranded DNA (dsDNA) on MGI and Illumina platforms. To simplify the experimental process, multiple processes were applied in one single step, including the fragmentation, end repair and adapter ligation. This A-T ligation-based kit applies to the whole genome sequencing with DNA input ranging from 5 to 500 ng, and is compatible with hybridization capture based targeted sequencing. Product details Product Feature ● Suitable for various types of samples including gDNA, FFPE B+/B/C/D DNA samples+ ● The length of DNA fragment is flexible and controllable； ● Flexible system and simple operation, with fragmentation, end repair and A-addition being completed in one step； ● Generate high quality libraries with uniform and unbiased coverage as well as low GC bias； ● Low background noise (abnormal sequence introduced by enzymatic digestion by-products)； ● Compatible with automatic workstations； Can RNA samples be directly used for library preparation with this kit? No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation. What is the recommended input range? How to handle inputs exceeding this range? This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency. What is the insert size of this kit? How to select sequencing read length? The main peak of PCR products by using this kit is ~270 bp.