Solution reagent kit NadPrep Methyl

for DNA library preparation

NadPrep Methyl Library Preparation Kit v2 is designed for preparation of high-quality methylation sequencing (Methyl-Seq) libraries from double-stranded DNA (dsDNA) on both Illumina and MGI platforms. This kit is suitable for preparing Methyl-Seq libraries from 1-500 ng DNA, supporting whole-genome bisulfite sequencing (WGBS) as well as hybrid capture-based targeted methylation sequencing with effective enrichment of targeted libraries. Feature Supports input amounts ranging from 1-500 ng across different DNA sample types, enabling better sample utilization. Compatible with various conversion methods and sequencing platforms, offering flexibility for diverse applications. Enables WGBS library preparation in as fast as 4.5 hr with bisulfite conversion. Significantly improves effective sequencing depth and data utilization. Can RNA samples be directly used for library preparation with this kit? No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation. What is the recommended input range? How to handle inputs exceeding this range? This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency. What is the insert size of this kit? How to select sequencing read length? The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.