Solution reagent kit 1002701

for DNA library preparationfor DNA

NadPrep DNA Methyl Bisulfite Conversion Module is a methylation conversion reagent developed for DNA samples based on the principle of bisulfite conversion. The conversion module, based on a magnetic bead purification protocol, efficiently conv- erts unmethylated cytosine to uracil within 2 hr, leaving methylated cytosine unchanged, and it can be compatible with autom- ated workstations. It can be seamlessly integrated with Nanodigmbio's NadPrep double-strand methylation library preparation scheme and NadPrep single-strand methylation library preparation scheme, allowing flexible selection among various types of starting samples. Feature Compatible with multiple types of samples and supports a wide range of input amounts Efficient and stable cytosine conversion rate Excellent detection sensitivity of 5mC and 5hmC Convenient operation is conducive to automation implementation Can RNA samples be directly used for library preparation with this kit? No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation. hat is the recommended input range? How to handle inputs exceeding this range? This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency. What is the insert size of this kit? How to select sequencing read length? The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.