Solution reagent kit NadPrep

NGSFFPE tissues

NadPrep Total RNA-To-DNA Module is a reverse transcription module developed for NGS platform. This module offers an efficient solution for the generation of dsDNA fragments from 10-200 ng of Human total RNA from cells, tissues and FFPE samples.The dsDNA output can couple with the NadPrep DNA Library Preparation kit. Product details Features Versatile: Compatible with multiple RNA samples, including cells, fresh tissues and FFPE tissues. High Performance: Ensure dsDNA library output with high efficiency and stability. Friendly Workflow: The dsDNA output can directly couple with the NadPrep DNA Library Preparation kit without fragmentation steps. Can RNA samples be directly used for library preparation with this kit? No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation. What is the recommended input range? How to handle inputs exceeding this range? This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency. What is the insert size of this kit? How to select sequencing read length? The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage. What is the recommended sequencing data volume for IGTR immune repertoire analysis? A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng. Increase data volume to enhance sensitivity for low-frequency clones.