Solution reagent kit 1002241

for DNA library preparation

The NadPrep Universal Circularization Kit v2 is designed for preparation of a single-stranded circular DNA (ssCirDNA) library for the MGI platform based on DNBSEQTM technology. The circularization module inside is compatible with the circularization of libraries with single index (SI) adapters and dual index (DI) adapters on MGI platform, and NadPrep Universal Stubby Adapters (UDI), and is suitable for the circularization of libraries converted by the MGIEasy Universal Library Conversion Kit (App-A), with the preparation of single-stranded circular libraries effectively streamlined. Feature ● Wide applicability: applicable to many types of library ● High flexibility: compatible with libraries of various types, insert fragment size and input amounts ● Efficient circularization: high circularization efficiency and stability ● Optimized workflow: 1/3 shorter experiment time compared to v1 Can RNA samples be directly used for library preparation with this kit? No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation. What is the recommended input range? How to handle inputs exceeding this range? This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency. What is the insert size of this kit? How to select sequencing read length? The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.