Solution reagent kit μCaler

for RNA library preparationliquidSARS-COV-2

μCaler Hybrid Capture Reagents that have been optimized for targeted enrichment of small panels and, coupled with μCaler NanoBlockers and μCaler Panel based on Nanodigmbio’s independent intellectual property rights with innovative scheme design have constituted a complete liquid hybridization capture system, thus enabling the whole process completion of capture-library preparation in same day. The μCaler SARS-Cov-2 Panel is designed based on the Wuhan-Hu-1/2019 (MN908947) reference genome with dependence on the μCaler probe design scheme. It covers all 29.9 Kb genomic regions and can be used for precise typing, variant tracking, and identification of new variants of SARS-CoV-2. Features ● Simplified process for easier operation ●Complete the whole process from samples to capture-library preparation in same day ● Small panel has stable and excellent capture performance Can RNA samples be directly used for library preparation with this kit? No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation. What is the recommended input range? How to handle inputs exceeding this range? This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency. What is the insert size of this kit? How to select sequencing read length? The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.