Solution reagent μCaler

for hybridization

μCaler MRD Solution is equipped with adapters containing dual Unique Molecular Identifiers (UMI & BMI), so as to meet the analysis requirements of ultra-low frequency mutations in plasma circulating cell-free DNA for MRD detection; in addition, this solution is designed for targeted enrichment of small Panel, integrated with upgraded and optimized hybrid capture and elution processes, and equipped with μCaler Panel designed based on innovative protocols, which can complete the whole process of capture-library preparation in same day. Schematic Diagram of Working Principle in μCaler Hybrid System Find target region by probe to form stable binding Rapid binding of multiple probe conjugation effects for further stabilization Feature Simplified process for easier operation More Faster for hybridization capture,Complete the whole process from samples to capture-library preparation in same day Small Panel has stable and excellent capture performance Compatible with automated workstations Can RNA samples be directly used for library preparation with this kit? No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation. What is the recommended input range? How to handle inputs exceeding this range? This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency. What is the insert size of this kit? How to select sequencing read length? The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.