Solution reagent kit μCaler HotSpot Panel v1.0

for researchfor PTEN genefor TP53 gene

utation, involving 49 genes related to carcinogenesis and tumor suppressor, with probes covering approximately 25 Kb of the genome. This Panel is designed for use with the μCaler Hybrid Capture System. Based on the novel μCaler targeted enrichment system, the μCaler HotSpot Panel v1.0 delivers improved capture efficiency, consistent and excellent capture performance, and sig- nificantly reduces experimental time, enabling more efficient sequencing and cost savings. Can RNA samples be directly used for library preparation with this kit? No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation. What is the recommended input range? How to handle inputs exceeding this range? This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency. What is the insert size of this kit? How to select sequencing read length? The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage. hat is the recommended sequencing data volume for IGTR immune repertoire analysis? A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng. Increase data volume to enhance sensitivity for low-frequency clones. Is this kit suitable for minimal residual disease (MRD) monitoring? Yes. This kit includes IG Primer Mix and TR Primer Mix with gene-specific primers provided in separate tubes, allowing flexible combination in a single amplification reaction.