Solution reagent kit NadPrep

for research

platforms.The NadPrep NanoBlockers act by reducing non-specific binding of adapter sequences, which improves on-target rate and increases enrichment depth. NadPrep NanoBlockers(for MGI, SI) only work with MGI libraries with 10nt single indexes (SI). NadPrep NanoBlockers(for MGI, DI) only work with MGI libraries with 10 nt dual indexes (DI),They are non-interchangeable. Features Universal blockers for MGI platforms. The NadPrep NanoBlockers (SI) and the NadPrep NanoBlockers (DI) work with MGI libraries with 10 nt single indexes and dual indexes respectively. Effectively reduce non-specific binding of adapter sequences and significantly increase the on-target rate and increase enrichment depth. Can RNA samples be directly used for library preparation with this kit? No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation. What is the recommended input range? How to handle inputs exceeding this range? This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency. What is the insert size of this kit? How to select sequencing read length? The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage. What is the recommended sequencing data volume for IGTR immune repertoire analysis? A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng. Increase data volume to enhance sensitivity for low-frequency clones.