Solution reagent kit NadPrep ES

for hybridizationNAD

NadPrep ES Hybrid Capture Reagents is a kit optimized for the targeted capture with rapid hybridization elution steps of NAD Panels/Probes developed by Nanodigmbio. The complete liquid-phase hybridization capture system is composed of NadPrep NanoBlockers series and 120 nt NAD Panels/Probes series, and supports flexible selection between 0.5-4 hr rapid hybridization and overnight hybridization by adjusting the hybridization time to optimize the capture performance for panels of different sizes. Feature ● Simplify the types of elution buffer into one to reduce the possibility of human errors during operation ● Simplify the experimental process, make it more convenient and user-friendly for operation to facilitate the development of automatic workstation. ●Support flexible selection between rapid hybridization for 0.5-4 hr or overnight hybridization for 16 hr. ● Support the adjustment of hybridization time to optimize capture performance. Can RNA samples be directly used for library preparation with this kit? No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation. What is the recommended input range? How to handle inputs exceeding this range? This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency. What is the insert size of this kit? How to select sequencing read length? The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.