Solution reagent NEXome Plus Panel v1.0

for researchcalibrationMET

NEXome Plus Panel v1.0 is an enhanced whole exome capture Panel. It extends the target regions to SNP skeleton of whole genome, intronic regions related to common gene fusion in solid tumors and classical microsatellite loci on the basis of NEXome Core Panel, which spans a 43.3 Mb region of human genome. SNP skeleton of whole genome Based on the 1000 Genomes database, we mainly select >9,000 loci with high MAF values and high heterozygosity in the Chinese population, covering the entire human genome at approximately 300 Kb intervals. It provides richer and more evenly distributed information on the genomic composition based on the whole exome, and can be used for detection of CNVs and loss of heterozygosity. Classical microsatellite loci The NEXome Core Panel itself covers a large number of microsatellite loci, and the addition of 15 optimally designed MSI classical loci facilitates the comparison between different microsatellite instability analytical methods as well as the optimization and calibration of the analytical process. Can RNA samples be directly used for library preparation with this kit? No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation. What is the recommended input range? How to handle inputs exceeding this range? This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency. What is the insert size of this kit? How to select sequencing read length? The main peak of PCR products by using this kit is ~270 bp.