Solution reagent NEXome

On the basis of the latest version of Refseq (109, 2021) and the CCDS database, NEXome Core Panel has carefully selected some regions that are worthy of attention outside the database. At the same time, based on product continuity considerations, some egions deleted in the latest version of the database have been retained. The NEXome Core Panel contains approximately 400,000 single-stranded DNA probes by independently synthesized and independently inspected for quality, targeting a 34.7Mb genomic region (19,613 genes). NEXome Core Panel, as a core all-exome Panel which can be combined with different spike-in panels to meet different application requirements. Production and Quality Control Independently Synthesized and Independently Inspected for Quality Can RNA samples be directly used for library preparation with this kit? No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation. What is the recommended input range? How to handle inputs exceeding this range? This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency. What is the insert size of this kit? How to select sequencing read length? The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage. What is the recommended sequencing data volume for IGTR immune repertoire analysis? A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng. Increase data volume to enhance sensitivity for low-frequency clones.