Solution reagent kit NanOnco Plus Panel v3.0

for researchdiagnosticfor HLA-B gene

NanOnco Plus Panel v3.0 targets the full coding regions from 620 genes (HLA genes included) of interest in solid tumor studies, a series of intron regions related to common fusions, classical microsatellite sequences, and chemotherapy-associated polymorphic loci. This panel involves a total number of 637 genes, covering an approximate 2.4 Mb target region of the genome. It supports the enrichment of multiple variants including base substitution, insertion/deletion, gene rearrangement, gene amplification, microsatellite instability and so on. Can RNA samples be directly used for library preparation with this kit? No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation. What is the recommended input range? How to handle inputs exceeding this range? This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency. What is the insert size of this kit? How to select sequencing read length? The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage. What is the recommended sequencing data volume for IGTR immune repertoire analysis? A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng. Increase data volume to enhance sensitivity for low-frequency clones.