Solution reagent kit HiSNP

for researchSNP

HiSNP Ultra Panel v1.0 is designed for analyzing genomic composition status with high resolution. This SNP skeleton-based panel can scan the whole genome with 50 Kb apart by targeting over 52,000 high MAF SNP sites. HiSNP Panel v1.0 is designed for analyzing genomic composition status with basic resolution. This SNP skeleton-based panel can scan the whole genome with 300 Kb apart by targeting over 9,000 high MAF SNP sites. Feature High heterozygosity ratio: Comprehensive designing for Europeans, Africans, Americans and Asians (AF=0.05-0.95) Stable capture performance: GC balanced; unique flanking sequence (verified by WGS) Appropriate proportionality: Even distribution in whole genome; LD sites excluded Superior compatibility: Use alone or spike-in other CDS panels Can RNA samples be directly used for library preparation with this kit? No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation. What is the recommended input range? How to handle inputs exceeding this range? This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency. What is the insert size of this kit? How to select sequencing read length? The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage. What is the recommended sequencing data volume for IGTR immune repertoire analysis? A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng.