Solution reagent kit Ext-RNA

for researchfor genes

The ERCC RNA Spike-in Control Mixes is developed by National Institute of Standards and Technology (NIST) which is composed of a serious of RNA sequences with determined concentrations. It can derive standard curves relating read counts to RNA concentration and is widely used in performance assessment of gene expression experiments. Ext-RNA Control Panel v1.0 targeted 24 of 92 ERCC transcripts 273-2,022 bp in length, with concentration ranging from 0.014305 attomol/μL to 937.5 attomol/μL. The 24 ERCC RNAs will serve as external controls for RNA expression quantification. Can RNA samples be directly used for library preparation with this kit? No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation. What is the recommended input range? How to handle inputs exceeding this range? This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency. What is the insert size of this kit? How to select sequencing read length? The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage. What is the recommended sequencing data volume for IGTR immune repertoire analysis? A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng. Increase data volume to enhance sensitivity for low-frequency clones. Is this kit suitable for minimal residual disease (MRD) monitoring? Yes. This kit includes IG Primer Mix and TR Primer Mix with gene-specific primers provided in separate tubes,