Solution reagent kit

NGSquality controlNAD

NAD Probe is a one-stop service created by Nanodigmbio, covering the entire process from probe design, probe production, quality inspection, optimization, and result delivery. Through strict quality control (QC) and optimized NGS QC services, we can provide our customers with high-quality customized hybridization capture solutions, covering the application types in addition to conventional mutation detection, which include methylation capture, RNA capture, and virus capture. Features Clarified sequence and known concentration for each probe In compliance with the ISO13485 system production standard, providing uniform and stable results Good uniformity of coverage, low GC bias Support spike-in to other NadPanels or customized panels Support hybridization capture of multiple samples Can RNA samples be directly used for library preparation with this kit? No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation. What is the recommended input range? How to handle inputs exceeding this range? This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency. What is the insert size of this kit? How to select sequencing read length? The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage. What is the recommended sequencing data volume for IGTR immune repertoire analysis? A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng.