Solution reagent kit NadPrep DNA Methyl Bisulfite Conversion Module

NGSfor DNA library preparationfor DNA

NadPrep DNA Methyl Bisulfite Conversion Module is a methylation conversion reagent developed for DNA samples based on the principle of bisulfite conversion. The conversion module, based on a magnetic bead purification protocol, efficiently conv- erts unmethylated cytosine to uracil within 2 hr, leaving methylated cytosine unchanged, and it can be compatible with autom- ated workstations. It can be seamlessly integrated with Nanodigmbio's NadPrep double-strand methylation library preparation scheme and NadPrep single-strand methylation library preparation scheme, allowing flexible selection among various types of starting samples. Feature： -Compatible with multiple types of samples and supports a wide range of input amounts -Efficient and stable cytosine conversion rate -Excellent detection sensitivity of 5mC and 5hmC -Convenient operation is conducive to automation implementation -Can RNA samples be directly used for library preparation with this kit? This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation. This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency. The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.