The ERCC RNA Spike-in Control Mixes is developed by National Institute of Standards and Technology (NIST) which is composed of a serious of RNA sequences with determined concentrations. It can derive standard curves relating read counts to RNA concentration and is widely used in performance assessment of gene expression experiments.
Ext-RNA Control Panel v1.0 targeted 24 of 92 ERCC transcripts 273-2,022 bp in length, with concentration ranging from 0.014305 attomol/μL to 937.5 attomol/μL. The 24 ERCC RNAs will serve as external controls for RNA expression quantification.