Albumin reagent 1183GR025

for electrophoresisliquidprotein

Albumin Fraction V, also named Bovine Serum Albumin (BSA, bovine albumin), is a protein extracted from bovine blood plasma. The designation "Fraction V" is based on Cohn's extraction method, which takes advantage of the different solubility behavior of the proteins in the blood plasma. In the separation of plasma proteins performed according to Cohn, BSA represents the fifth fraction. BSA shows a net negative charge under physiological conditions and is a small (~67 kDa), stable protein. In blood, BSA regulates colloid osmotic pressure and acts as a transport protein for 2-valent cations (Zn2+, Cu2+). In addition, bovine albumin shows high affinity for polar (water, salts) and nonpolar substances (e.g. fatty acids, hormones). It is also able to bind toxic and pyrogenic substances, making it a valuable additive in cell culture media. BSA is also the main component of fetal calf serum (FBS). However, BSA is not a substitute for FBS because the numerous components of FBS in their complex properties cannot be replaced by a single protein. Albumin Fraction V is available in different grades suitable for different applications depending on their properties/parameters. Based on the extraction and purification process that is used, the final BSA product will show differences in purity and amount of traces of enzymes, metabolites, peptides, fatty acids, etc. Applications The applications of BSA are extremely diverse. It is a common additive in cell culture media and solutions (as growth factor, stabilizer, detoxifier) and an essential component in a wide variety of biochemical and immunological applications.