BioKits BLG Assay Kit is used for the detection and quantification of β-lactoglobulin (BLG) in food products and environmental swabs by enzyme immunoassay.
Specifications
Analyte - BLG
Platform - ELISA
Result Type - Quantitative
Limit of Detection - 2 ppm BLG ppm BLG
Testing Time - 120.00
Cross Reactivity - Not suitable for testing 100% egg products (raw or powder). This includes meringue based products where egg powder content is very high. In general where egg is an ingredient of a final product intended for consumption there is no cross-reactivity evident.
Prop 65 Warning WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm.
Quantity per Package - 36
Package Weight - 1.00 lb
Materials Provided
Milk Protein Standard
BLG Control
BLG Biotin
Avidin Peroxidase Conjugate
TMB Substrate
Wash Solution Concentrate
Diluent Concentrate Type 10
Stop Solution
BLG Plate
Package Insert
Worksheet
Materials Required But Not Provided
0.05M carbonate / bicarbonate buffer pH 9.6 OR equivalent buffer tablets e.g. Sigma C3041
Purified water
Fish gelatin (Sigma G7765) optional
Ultra Turrax or similar homogenizer, and/or Waring or similar blender or food processor, and/or stomacher or similar homogenizer
Miscellaneous laboratory plastic and/or glassware, including measuring cylinders, pipettes, disposable Pasteur pipettes, plate seals and containers suitable for food extracts
pH meter
Water bath capable of maintaining 60°C ± 2°
Wrist action or similar shaker
Validated microwell plate shaker set at 700 – 800 rpm