Quality control test kit 103719-1

for forensic applicationsbloodurine

Highly sensitive Multiple matrices Large menu of tests available Neogen's Bumetanide ELISA (Enzyme-Linked ImmunoSorbent Assay) kit is a qualitative one-step kit designed for use as a screening test for the detection of Bumetanide and/or their metabolites. The kit was designed for screening purposes and is intended for forensic use only. Specifications - Intended Use - For the determination of trace quantities of Bumetanide and/or other metabolites in human urine, blood or oral fluid. Contact a Neogen representative for information on other matrices. Result Type - Qualitative Microplate Size - 96 well microplate Sensitivity The term I-50 is used todefine the sensitivity of the test. This number is derived from a standard curve generated with the drug in EIA Buffer. The drug concentration that shows 50% less color activity than the zero standard is considered to be the I-50. Storage Conditions - Test kit 2-8°; store controls -20°C Wavelength - 650 nm with Red Stop Solution 450 nm with acid stop Package Weight - 0.70 lb Cross Reactivity Compound - % Cross Reactivity Bumetanide - 100% Materials Provided EIA Buffer Wash Buffer Concentrate (10X) K-Blue Substrate Drug-Enzyme Conjugate Antibody Coated Plate Red Stop Solution Qualitative QC Positive Control Qualitative QC Negative Control Materials Required But Not Provided Deionized water. Graduated cylinder to dilute and mix wash buffer. Plate cover or plastic film to cover plate during incubation. Clean glassware (i.e. test tubes) to dilute samples. Microplate reader with 650 nm filter. Cut-off calibrator Optional Test Materials Qualitative QC Positive Control: 5 x750 μL (synthetic human urine)