Highly sensitive
Multiple matrices
Large menu of tests available
Neogen’s Ethacrynic Acid ELISA (Enzyme-Linked ImmunoSorbent Assay) kit is a qualitative one-step kit designed for use as a screening test for the detection of Ethacrynic acid. The kit was designed for screening purposes and is intended for forensic use only.
Platform - ELISA
Result Type - Qualitative
Microplate Size - 96 well microplate
Intended Use - For the determination of trace quantities of Ethacrynic Acid and/or other metabolites in human urine, blood or oral fluid. Contact a Neogen representative for information on other matrices.
Sensitivity
The term I-50 is used to define the sensitivity of the test. This number is derived from a standard curve generated with the drug in EIA Buffer. The drug concentration that shows 50% less color activity than the zero standard is considered to be the I-50.
Storage Conditions - Test kit 2-8°; store controls -20°C
Wavelength -
650 nm with Red Stop Solution
450 nm with acid stop
Package Weight - 0.70 lb
Cross Reactivity
Compound - % Cross-Reactivity
Ethacrynic Acid - 100%
Methylene Blue - 8%
Materials Provided
EIA Buffer
Wash Buffer Concentrate (10X)
K-Blue Substrate
Drug-Enzyme Conjugate
Antibody Coated Plate
Red Stop Solution
Qualitative QC Positive Control
Qualitative QC Negative Control
Materials Required But Not Provided
Deionized water.
Precision pipettes that range from 10 μL - 1000 μL and disposable tips.
Graduated cylinder to dilute and mix wash buffer.
Plate cover or plastic film to cover plate during incubation.
Clean glassware (i.e. test tubes) to dilute samples.
Microplate reader with 450 nm filter.
Cut-off calibrator