STORM microscope N-STORM

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STochastic Optical Reconstruction Microscopy (STORM) reconstructs a super-resolution fluorescence image by combining precise localization information for individual fluorophores in complex fluorescent specimens. N-STORM takes advantage of Nikon's powerful Ti2-E inverted microscope and applies high-accuracy, multi-color localization and reconstruction in three dimensions (xyz) to enable super-resolution imaging at tenfold the resolution of conventional light microscopes (up to approximately 20 nm in xy). This powerful technology enables the visualization of molecular interactions at the nanoscopic level, opening up new worlds of scientific understanding. In addition to lateral super-resolution, N-STORM utilizes proprietary methods to achieve a tenfold enhancement in axial resolution over conventional light microscopes and provide nanoscale information in 3D. The 3D-Stack function allows multiple 3D STORM images from different Z positions to be captured and stitched into one image to create thicker STORM images. Tenfold improvement of lateral resolution up to 20nm N-STORM utilizes high accuracy localization information for thousands of individual fluorophores present in a field of view to create breathtaking "super-resolution" images, exhibiting spatial resolution that is 10 times greater than conventional optical microscopes. Dynamic super-resolution imaging Newly developed optics and illumination systems, optimized for sCMOS technology, have increased image acquisition speeds by up to 10 times.With acquisition times reduced from minutes to seconds*, dynamic events in live specimens can now be captured with molecular level resolution.