Reverse Complement (RC-PCR) based MPS library prep solution for Y-STR haplotyping, to characterise paternal lineages of unknown male trace donors.
RC-PCR provides multiplex targeted amplification, sequencing indexing and adaptor addition occuring simultaneously in a single closed tube reaction.
Compatibility
All amplicons are designed to meet the shortest possible fragment lengths and are compatible with illumina® MiSeq™ systems, with 2x 10 bp Unique Dual Index reads.
IDseek® Kits have been tested with Tannic Acid and Humic Acid. RC-PCR technology allows the reliable analysis of highly degraded samples frequently found in forensic casework.