BrilliantDye™ Terminator (v1.1) Cycle Sequencing Kit provides the reagents (BrilliantDye Terminator, 5x sequencing buffer, pGEM control, M13 control primer) required for Sanger Sequencing reactions, in a ready for use pre-mixed format. It is optimized to provide a robust and highly flexible chemistry, designed for all kinds of DNA sequencing applications, including de novo and resequencing.
BrilliantDye™ Terminator (v1.1) Cycle Sequencing Kit is conveniently offered in low to medium to high-throughput packaging, ranging from 24, 100, 1,000, and 5,000 to 25,000 reactions respectively.
Compatibility
BrilliantDye™ Terminator Cycle Sequencing kits and associated buffers are widely adopted as proven, high-quality reagents for laboratories using 310, 3100, 3130, 3500, 3730 Series Genetic Analyzers and SeqStudio™.
BrilliantDye™ is developed for performing fluorescence-based cycle sequencing reactions on single-stranded or double-stranded DNA templates, including PCR fragments and plasmids. BrilliantDye™ generates data with uniform peak heights and optimized signal balance to produce long, high-quality reads, as well as highly accurate base assignments.
BrilliantDye™ Terminator v1.1 Cycle Sequencing Kits are a drop-in replacement for BigDye® Terminator v1.1 Cycle Sequencing Kits without having to change protocol, volume, or setting. The products can be used with other products in the Sanger sequencing workflow (e.g. NimaPOP™ or POP™ polymers and buffers) on Applied Biosystems® 3130, 3500, 3730 and SeqStudio™ Flex Genetic Analyzers.
BrilliantDye and NimaPOP are trademarks of NimaGen, B.V. SeqStudio and POP are a trademark of Thermo Fisher Scientific, Inc.