Enzyme reagent FastGene® apTaq
for molecular biologyfor nucleic acidshot start

enzyme reagent
enzyme reagent
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Characteristics

Type
enzyme
Applications
for molecular biology, for nucleic acids
Other characteristics
hot start

Description

DNA-Polymerase with aptamer technology: Reversible enzyme activation Advantages Hot start: Very fast PCR Aptamer technology: Reversible enzyme activation or inactivation Maximal specificity, sensitivity and yield Robust and reliable reaction Tolerate a wide range of templates apTaq HotStart Polymerase – Redefine your PCR The FastGene® apTaq DNA-Polymerase is a recombinant and thermostable Taq-Polymerase using the aptamer based Hot Start activation technology. The aptamer allows a reversible and immediate activation of the polymerase, leading to specific priming and a very fast PCR. Reversible Polymerase activation – The aptamer principle The FastGene® apTaq DNA-Polymerase is a Hot Start enzyme, which is completely inactive during room-temperature and become active only after heating. Random primer annealing and unspecific amplification that can occur with standard PCR enzymes are problems of yesterday. In contrast to antibody-based methods, the apTaq DNA-Polymerase contains synthetically manufactured aptamer-oligonucleotides. At low temperatures the polymerase is inactivated through a reversible tight bond of the aptamer. Interestingly, the aptamer acts as a molecular switch, changing its tertiary structure at higher temperatures. Temperatures below 45° C inactive the polymerase, whereas temperatures above 60 °C fully activated the enzyme. Therefore, the FastGene® apTaq DNA-Polymerase is less temperature-sensitive and reduce the risk of contamination.

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.