For rapid and simple purification of all sizes of cell-free circulating DNA (cfc-DNA) and circulating tumour DNA (ct-DNA) from plasma and serum samples
Versatile plasma and serum input volumes (0.5 mL - 6 mL)
Minimal high molecular weight gDNA contamination in the purified cfc-DNA
Isolate inhibitor-free cell-free circulating DNA
Compatible with fresh, preserved or frozen serum/plasma prepared from blood collected on either Norgen's cf-DNA/cf-RNA Preservative Tubes (Cat. 63950, 63960), Cell-Free DNA BCT ® (Streck), Heparin, EDTA or Citrate
Fully automated Isolation procedure on Hamilton MicroLab Nimbus
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Plasma/Serum cfc-DNA Advanced Purification Kit
Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit provides fast, reliable and simple procedures for isolating the highest quality and quantity of cell-free circulating DNA (cfc-DNA) from various amounts of plasma/serum ranging from 1 mL up to 6 mL with minimal genomic DNA (gDNA) contamination. Isolation is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all sizes of cfc-DNA and ct-DNA from either fresh, preserved or frozen plasma/serum samples.The purified plasma/serum cfc-DNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.