NGS reagent kit 733 series

reagent mediumproteinase Kprotease

For the purification of exosomes and the subsequent isolation of RNA from different cell culture media sample volumes up to 35 mL Overview Ensure optimal results for sensitive applications like NGS Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs Purification and enrichment of intact cell culture media exosomes for functional studies No phenol extractions, Proteinase K treatment, nor carrier RNA required No time-consuming ultracentrifugation, filtration nor special syringes required No precipitation reagents nor overnight incubation required Pure exosomes are purified and are free-from any other RNA-binding proteins Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix Norgen’s EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allows the user to elute into flexible elution volumes ranging from 50 μL to 100 μL.