Buffer solution reagent kit NZYMaxiprep

resindiagnosticfor DNA library preparation

NZYMaxiprep kit allows for rapid preparation of large-scale highly pure plasmid DNA from E. coli strains. Details NZYMaxiprep kit is designed for the rapid, large-scale preparation of highly pure plasmid DNA (typically 500 µg) from recombinant Escherichia coli strains. Plasmid DNA binds selectively to NZYTech columns charged with a silica-based anion-exchange resin. All contaminants, such as proteins, RNA, salts, nucleotides and oligos (<40-mer) are washed from the column. In the elution step, the positive charge of the resin is neutralized by a pH shift to slightly alkaline conditions and pure plasmid DNA is eluted in a high-salt elution buffer. To isolate DNA from low copy number plasmids, BACs or cosmids, or to obtain higher DNA concentrations, use 500 mL of E. coli cultures and double the volumes of Buffers M1, M2 and M3. Shipping Conditions - Room Temperature Application - In vitro transcription, Cloning, PCR, Sequencing, Transformation Scale - Maxiprep Features - Manual, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number Format - Column Product Category - Plasmid DNA Purification Isolation Technology - Silica membrane Protocol time - Approx. 90 min