Flu test kit MD04851

COVID-19for genesinfluenza A

NZYTech´s COVID-19 & Flu A/B Multiplex One-Step RT-qPCR Kit, IVD provides the complete set of reagents and probes to qualitatively detect the SARS-CoV-2 and/or Influenza genomes, through common real-time PCR platforms.SARS-CoV-2 is identified by detecting RT-qPCR targets located in RdRp and N genes. In contrast, Influenza A and Influenza B viruses are detected through the amplification of targets located in M1 and NS2 genes, respectively. NZYTech´s COVID-19 & Flu A/B Multiplex One-Step RT-qPCR Kit, IVD is built to have the broadest detection profile possible whilst remaining specific to the SARS-CoV-2 and Influenza types A and B genomes. NZYTech’s COVID-19 & Flu A/B Multiplex One-Step RT-qPCR Kit, IVD is a multiplex assay detecting SARS-CoV-2, Influenza A, Influenza B and Human nucleic acids (acting as an internal positive control). Extracted and purified RNA is transcribed to cDNA and subsequently amplified in a single reaction using five highly specific primers/probe sets, namely those detecting SARS-CoV-2 RNA dependent RNA polymerase (RdRp) and the Nucleocapsid phosphoprotein (N) genes, Influenza A and B Matrix (M1) and Nonstructural 2 (NS2) specific genes and the human ribonuclease P (RNase P, RP) gene. The kit exploits the so-called TaqMan® principle. During this process, probes specifically anneal to their target genes and upon DNA amplification, through two flanking primers, are subjected to degradation leading to the separation of the reporter dye from the quencher thus resulting in an increase in fluorescence.