Reverse transcriptase reagent kit MB40901

for RT-PCRfor cDNA synthesisRT-qPCR

Lyo NZY Reverse Transcriptase, provided freeze-dried, synthesizes cDNA strand in the presence of a primer using either RNA or single-stranded DNA as template in a wide range of temperatures (37-50°C). Details Lyo NZY Reverse Transcriptase is a freeze-dried enzyme provided with an optimized reconstitution buffer designed to confer maximal levels of stability and enzymatic activity. Lyo NZY Reverse Transcriptase is a modified recombinant form of the Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase purifiedfrom Escherichia coli. Lyo NZY Reverse Transcriptase synthesizes the complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template in a wide range of temperatures (37-50°C). High reaction temperatures are beneficial to improve specificity and to allow synthesis of cDNA from complex templates, including those with high GC-content or with high degree of secondary structure. The enzyme lacks 3´→5´ exonuclease activity and has no RNase H activity, enabling improved synthesis of full-length cDNA even for long mRNA, using random priming. Thus, Lyo NZY Reverse Transcriptase gives high yields of cDNA up to 7 kb. Lyo NZY Reverse Transcriptase can be used in first-strand cDNA synthesis experiments, RT-PCR, RT-qPCR, DNA labelling and analysis of RNA by primer extension. Components – Lyo NZY Reverse Transcriptase (100000 U) – Lyo NZY-RT resuspension buffer – Reaction Buffer (10x) – 0.1 M DTT More Information Shipping Conditions Room Temperature Features Fast, High yield, Thermostable Reaction Temperature 50 °C Heat Inactivation 85 °C Protocol time Approx. 60 min