Proteinase K reagent kit NZY Mag

nucleic aciddiagnosticfor qPCR

NZY Mag Viral RNA/DNA Isolation kit, IVD, is a magnetic bead technology‑based nucleic acid purification kit designed to recover RNA and DNA from viral particles contained in transport medium from human nasopharyngeal swabs. The kit uses magnetic-bead technology to provide reproducible recovery of high-quality nucleic acids. The procedure is based on the reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions. Sample lysis and binding to the NZY Mag Binding Beads is achieved by incubation with NZY Mag Binding Buffer containing chaotropic ions supported by nucleic acid release with NZY Mag Proteinase K digestion. Following nucleic acid magnetic separation, paramagnetic beads holding the RNA/DNA are washed to remove contaminants and salts using NZY Mag Wash Buffer and 80% ethanol. Residual ethanol from previous wash steps is removed by air-drying. Finally, highly pure viral RNA/DNA is eluted with NZY Mag Elution Buffer. It is highly recommended to use extraction controls, positive and negative controls, internal controls as well as to perform cross-contamination assays during the usage of NZY Mag Viral RNA/DNA Isolation kit, IVD. Protocol options for NZY Mag Viral RNA/DNA Isolation Kit, IVD, support automated workflows in a variety of platforms, including the Thermo ScientificTM KingFisher® Flex Magnetic Particle Processor with a 96 deep-well head. Automated workflows allow for 96 nasopharyngeal swab specimens to be processed in <30 minutes.