COVID-19 test kit MD0491 series

infectionfor genesSARS-COV-2

NZYTech SARS-CoV-2 One-Step RT-qPCR Kit III, 5 Targets, IVD, provides the complete set of reagents and probes to qualitatively detect the SARS-CoV-2 genome, through common real-time PCR platforms. The virus RNA dependent RNA polymerase (RdRp) and the Nucleocapsid phosphoprotein (N) genes have previously been identified as highly specific markers for SARS-CoV-2. In addition, this kit detects a third region located in structural envelope gene E, common to all Sarbecovirus and that is also present in SARS-CoV-2. This NZYTech kit targets specific regions in the RdRp, N and E genes of SARS-CoV-2 genome to provide the highest sensitivity of detection. One-step RT-qPCR remains the most reliable and sensitive method to perform an accurate detection of SARS-CoV-2 RNA, which is indicative of a human infection. Viral RNA isolated and purified from infected samples is retrotranscribed to cDNA and subsequently amplified in a single reaction using two highly specific primers/probe sets exploiting the so-called TaqMan® principle. During this process, the probes specifically anneal to five regions of the SARS-CoV-2 genome. An additional primers/probe set acts as an endogenous internal control to detect nucleic acids of the human RP gene, assessing sample quality. To allow identifying amplification of the six specific targets in a single reaction, SARS-CoV-2 RdRp, N & E genes and human RP specific probes are differently labelled, with HEX™, FAM™, Texas Red ® and Cy5™ reporter dyes, respectively. Note that this panel contains an hexaplex assay in four distinct optical channels which allow compensating emerging SARS-CoV-2 mutations and variants, ensuring confidence in your results.