NZY Tissue gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from a variety of sample sources including animal cells and tissues, Gram-positive and Gram-negative bacteria, mouse tails, yeast, forensic samples and clinical samples.
The method is spin column silica-based and requires no phenol or chloroform extraction. This kit uses optimized lysis buffers containing Proteinase K and SDS to release DNA from cells. After preparing the lysate, DNA is selectively absorbed into the NZYSpin Tissue Column and others impurities such as proteins and salts are removed during the washing steps. The eluted genomic DNA has a A260/280 ratio between 1.7 and 1.9 what makes it ready to use in applications like sequencing, PCR, multiplex-PCR, genotyping and a wide range of other enzymatic manipulations.
More Information
Shipping Conditions
Room Temperature
Application
Genotyping, PCR, qPCR, Sequencing
Starting Material
Animal cells, Animal Tissue, Body fluids, Buccal swabs, Forensic samples, Gram-negative bacteria, Gram-positive bacteria, Mouse tails, Paraffin embedded tissue, Plasma, Serum, Stool, Whole blood, Yeast
Scale
Miniprep
Features
Manual
Format
Column
Product Category
DNA Purification, Genomic DNA Purification
Isolation Technology
Silica membrane
Protocol time
Approx. 30 min