Solution reagent kit MD07351

clinicalRT-qPCRgenomic DNA

West Nile virus (WNV) is a single-stranded RNA virus that causes West Nile fever. It is a member of the family Flaviviridae, from the genus Flavivirus, which also contains the Zika virus, dengue virus, and yellow fever virus. The virus is primarily transmitted by mosquitoes and the primary hosts are birds. Humans and horses both exhibit disease symptoms from the virus. West Nile Virus RT-qPCR Kit is designed for the in vitro detection of West Nile Virus genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to WNV. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of WNV genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database at the time of design. Due to the inherent instability of RNA viral genomes, it is not possible to guarantee the detection of all clinical isolates. This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used. If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available for purchase(see Vet, Food & Pharma and Human).