Solution reagent kit MD06371

veterinary laboratoryfor qPCRlead

Neospora caninum is a coccidian parasite known to infect a variety of animals, particularly dogs and cattle. It is a leading cause of bovine abortion, causing economic losses in the cattle industry. In dogs, N. caninum can lead to neurological symptoms and reproductive issues, particularly when they consume infected tissues or are exposed during pregnancy. Neospora caninum qPCR Kit is designed for the in vitro detection of N. caninum genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to N. caninum. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of N. caninum genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected. This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used. If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available for purchase (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix.